Objective:To isolate and identify differential expression proteins in embryonic rat testis after maternal exposure to di-nbutyl-phthalate(DBP). Methods:Two-dimensional gel electrophoresis was performed on testes samples from hypospadiac and normal healthy rats. After silver staining,the differential expression proteins were analyzed by ImageMaster 2D analysis software. Results:Nine differential protein spots were found,calculated according to 5-fold expression amount. Five expressed highly in the hypospadiac rat testis but lowly in the normal rat testis,while the other four expressed just the opposite way. Conclusion:The protein profiles of differential expression between the normal and hypospadiac rat testes were established,and some differential proteins were found. The data obtained in this study might be helpful for further studies on the isolation and identification of differentially expressed proteins in hypospadiac rat testes.