Objective:To establish a cell model for ultraviolet damage to the skin and to study whether Eucommia has a protective effect against the UV-induced damage. Methods:Through the method of MTT,the most suitable Ultra Violet Radiation(UVR) time was screened out to best establish the model and found out the optimal concentration of Eucommia Ulmoides Oliv. Three groups were set in this experiments,control,UC and UE. UC were treated by a 30-minute-UVR,UE were treated by Extracts from Eucommia Ulmoides Oliv culture and then a 30-minute-UVR. Microscope and electromicroscope were used to morphologically observe the extent of damage by UVR. Further assessment was executed on the membrane potential of mitochondria and the content of ATP to better understand the extent of mitochondria damage. Results:30-minute-UVR was an ideal choice for the model. Extracts from Eucommia Ulmoides Oliv at the concentration of 0.4 mg/ml was demonstrated the most effective. Electromicroscrope showed cell would decline after a 30-minute-UVR. The protective effect on cell in UE group could be observed morphologically. The result of membrane potential of mitochondria was that UE declined by less extent than UC group compared with the control group.The ATP content ranging from high to low was UC,UE and control group. Conclusion:A damaged cellular model was established by 30 minutes of UV radiation,and the results showed the photoprotective function of eucommia at the concentration of 0.4 mg/ml.