Objective:To investigate the cytoskeleton reorganization of NRK52E during cellular ATP depletion and its mechanisms. Methods:We established a cellular ATP depletion model was established in vitro with 0.1 μmol/L antimycin A diluted in depletion buffer;F-actin was examined with fluorescence of FITC-phalloidin by flow cytometric analyses;the expression of ERM proteins was detected through Western Blotting and immunoblotting technologies. Results:The cellular ATP depletion model was established successfully,and the cellular ATP concentration declined in a time-dependent manner(P < 0.05); The F-actin of NRK52E was increasing during cellular ATP depletion,and these changes were paralleled with the severity of cellular ATP depletion(P < 0.05). When ATP was depleted,the ERM protein detached from the cytoskeleton into the cytoplasma. Conclusion:The cytoskeleton reorganization of NRK52E cells might be associated with the redistribution of ERM during ATP depletion.