Objective:To investigate the protective effects of QiShenYiQiJinGao on isoproterenol-induced chronic heart failure(CHF) and its effects on the myocardial energy metabolism in SD rats. Methods:142 male SD rats were randomly divided into two groups:control group and CHF group. Rats in CHF group were injected subcutaneously with isopreteronol(ISO). Four weeks later,the rats in CHF group were randomly re-divided into five groups:CHF model group,trimetazidine treated group,QiShenYiQiJinGao high,middle and low dose group. After an average treatment for five weeks,echocardiogram was performed to assess the cardiac function changes of these rats. The myocardial high energy phosphates and sarcoplasmic reticulum Ca2+-ATPase(SERCA) activity were determined by enzymatic analyses. Results:Compare with the model group,FS was significantly increased(31.48 ± 5.98 vs 36.35 ± 3.80,P < 0.05) in QiShenYiQiJinGao group. Meanwhile,EF also had a trend to be improved in QiShenYiQiJinGao group. Furthermore,the level of ATP and TAN(TAN=ATP+ADP+AMP) was increased by 19% and 31%,which was similar to the control group(P > 0.05). Compare with the model group,the SERCA activity was significantly increased in QiShenYiQiJinGao and trimetazidine treated group. Conclusion:QiShenYiQiJinGao could improve the cardiac function of ISO-induced CHF in SD rats via enhancing myocardial energy metabolism.