Objective:To evaluate the promoters’ activities of human interleukin-10(IL-10),IL-10 receptor(IL-10Rα),IL-4 and IL-4 receptor(IL-4Rα) during the lytic cycle replication of Kaposi’s sarcoma-associated herpesvirus induced by herpes simplex virus type 1 (HSV-1) in BCBL-1 cells. Methods:Promoters’ sequences of IL-10,IL-10Rα and IL-4Ra were amplified using PCR from total DNA of human genome. The PCR products were further cloned into pGL-3 basic vector to construct recombinant luciferase reporter plasmids. After infected by HSV-1,BCBL-1 cells were transfected with the promoter-driven luciferase constructs to induce luciferase gene expression and calculate the relative luciferase activity unit(RLU). Results:IL-10,IL-10Rα and IL-4Rα promoter sequences were isolated and constructed recombinant luciferase reporter plasmids successfully. The luciferase reporter recombinant exhibited a strong promoter activity,and the promoter-driven luciferase expression was increased significantly after infected with HSV-1 in BCBL-1 cells when compared with pGL-3 basic vector(P < 0.05). Conclusion:The data presented the evidence that HSV-1 could up-regulate the expression of IL-10 and IL-4 by activating IL-10,IL-4 and their receptors. And these data also suggested that IL-10 and IL-4 should partially contribute to HSV-1-induced KSHV replication in BCBL-1 cells.