Objective:To study the interleukin 13(IL-13) gene expression in T cells due to CD3/CD28 co-stimulation and the effect of arsenic trioxide(AT) on IL-13 gene expression. Methods:Hut-78 cells were stimulated with CD3/CD28 monoclonal antibodies(10 μg/ml and 5 μg/ml,respectively). IL-13 mRNA level in Hut-78 cells was measured in groups with or without AT treatment. Results:The level of IL-13 mRNA was increased in Hut-78 cells due to CD3/CD28 co-stimulation,however AT inhibited the gene expression of IL-13. Conclusion:AT would inhibit the gene expression of IL-13 in activated T cells,which might be one of its mechanisms reducing the airway inflammation in animal models.
HE Hai-yan,HUANG Mao,YAO Xin,DAI Shan-lin,SUN Pei-li,YIN Kai-sheng.Arsenic trioxide inhibits the gene expression of IL-13 in activated T cells[J].,2008,28(3):296-299.