Objective:To study the micromorphology and genetics of the muscular tissue from the female cadaver of Xi Han Dynasty discovered in Lianyungang in 2002. Methods:Light microscope and electron microscope were used to observe micro morph of the muscular tissue from the female cadaver,which had been fixed in 10% formalin after being unearthed. Besides,DNA Extract kits were used to extract genome DNA from muscular tissue;PCR was used to amplify mitochondrial DNA sequence. Results:Observed by both light microscope and electron microscope,the morph of the muscular tissue from the ancient cadaver sample was in a great mess. Genomic DNA(including mitochondrial DNA) of the cadaver was not obtained by extraction and amplification,respectively. Conclusion:The ancient cadaver fixed by formalin might be disadvantageous of the electron microscope and molecular biology research.
陈 芳,骆 莺,韩群颖,王建文,王林涛.连云港西汉女尸肌肉组织镜下形态学观察和DNA检测的探究[J].南京医科大学学报(自然科学版英文版),2008,28(3):335-337345.