Objective:To investigate the effect of epidermal growth factor receptor(EGFR) inhibitor AG1478 on adhesion,migration and invasion of HuH7 cell line and its molecular mechanism. Methods:HuH7 cells were treated with AG1478. Cell adhesion assay and Matrix-gel were used to examine the capability of adhesion,migration and invasion. Western blotting was employed to detect the expression of Erk2 and Paxillin in HuH7 cells. Results:AG1478(20 μmol/L) significantly inhibited the adhesion of HuH7 cells by 52%. And Matrix-gel experiment showed that AG1478(20 μmol/L) decreased the migration and invasion ability of HuH7 cells in vitro by 49% and 70%,respectively(P < 0.01). After the treatment of AG1478 for 24 h,the expression of Erk2 and Paxillin were decreased remarkably(P < 0.01). Conclusion:Our findings suggested that EGFR signal transduction pathway might play a key role on the capability of adhesion,migration and invasion in human HCC cell line HuH7,which could be partly related to the mechanism of Erk2 and Paxillin signal pathway.