Objective:To establish Livin specific gene-silencing system and to obtain stable clones of SGC-7901/siRNA-Livin. Methods:The small interfering RNA eukaryotic expression vector specific to Livin was constructed by gene recombination,and the nucleic acid was sequenced. Then it was transfected into SGC-7901 cells by Lipofectamin 2000. RT-PCR and Western blot were used to validate gene silencing efficiency of Livin in SGC-7901 cells. Stable clones were obtained by G418 screening. Results:Restriction endonuclease analyses showed that all plasmids had been successfully recombined. Nucleic acid sequencing showed that the siRNA was synthesized and inserted into pGPU6/GFP/Neo exactly. Livin silenced SGC-7901 cell clones were obtained after gene transfection and G418 selection,while the silencing efficiency of pGPU6/GFP/Neo/Livin-4 was higher than 70%. Conclusion:Livin specific siRNA was successfully constructed,and the expressions of Livin was silenced. Stable clones were obtained after gene transfection and screening.