Objective:To investigate the association between of JWA gene single nucleotide polymorphisms and the risk of cervical cancer. Methods:In a hospital-based case-control study of 227 cervical patients and 333 cancer-free controls frequency-matched by age,the genotype of the -76G>C,454C>A and 723T>G polymorphisms of JWA gene were detected by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method;the interaction of menarche age,reproductive age for the first time(RAFT) and parity were further analyzed. Results:A significant increased risk of cervical cancer was found to be associated with the -76GC genotype compared with the -76GG genotype(OR=1.84,95% CI=1.21~2.78),and 454AA genotype compared with the 454CC genotype(OR=1.84,95% CI=1.08~3.14). Moreover,individuals with 3~5 risk alleles will increase the risk of cervical cancer accompanied with early menarche age,late RAFT and many parity. There was no association found between JWA 723T>G polymorphism and cervical cancer. Conclusion:The JWA-76G>C and 454C>A polymorphisms might positively contribute to the etiology of cervical cancer.