Objective:To analyze the status of pathogenic bacteria distribution and drug resistance of hospital infection in emergency intensive care unit(EICU),for providing the guidance of clinical rational administration and decreasing the hospital infection. Methods:Pathogenic bacteria were isolated from the patients who suffered from hospital infection in EICU from January of 2006 to June of 2007. K-B slip diffusion method was taken to carry out the sensitive test . Rate of drug resistance of the pathogenic bacteria was analyzed. Results:The results showed that rate of the drug fast is very high. As the bacteria distribution ,the main pathogenic bacteria was the gram-negative bacilli(G-)(65.33 %), the second pathogenic bacteria was the gram-positive cocci(G+)(18.29%). and the mycetes(16.38%) occupied the third. In gram-negative bacilli the most were Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae and Escherichia coli;Staph is the main pathogenic bacteria in gram-positive cocci;and in mycetes the main pathogenic bacteria were Candida albicans and Candida glabrate. The detection rate of the extended spectrum β-lactamases(ESBLs) from the Escherichia coli and the Klebsiella pneumoniae was 58.06 % and 30.00%. The detection rate of the meticillin-resistant staphylococci(MRS) was 71.87%. Conclusion:Enhance monitoring on pathogenic bacteria distribution and drug resistance analyses of hospital infection in EICU could benefit for the guide of clinical rational administration,depressing multidrug-resistant bacteria and decreasing hospital infection.