Objective:To evaluate the therapeutic effect and indication for the treatment of lumbar disc herniation using intradiscal and paraspinal injection with O2-O3 mixture. Methods:One hundred and sixty-two patients were selected and divided into three groups by their category of the lumbar disc herniation which were verified by CT or MR imaging. For each herniated disc treatment,6~15 ml O2-O3 mixture at 40 ug/ml was injected into intradiscal space, and 10 ml of O2-O3 mixture were also injected into the paravertebral space when the needle withdrawned near the nerve root of the intervertebral forame,then the mixture of compound betamethason(1 ml), lidocaine(0.5 ml) and vitaminB12(0.5 mg) was injected. They were also divided into two groups with or without fibrous annulus,the correlation between fibrous annulus broken and therapeutic effect was studied. In addition,three patients with lumbar disc protrusion accompanied spinal stenosis were performed tentative ozone injection. Results:The patients were followed up from 6 months after treatment. The overall efficacy was 76.6%(141/162). Among them,31 cases(91.17%)in 34 bulging patients and 106 cases(89.07%) in 119 patients protrusion had excellent recovery;4 cases(44.44%)in 9 extrusion patients had significant relief of symptoms;63 cases(87.5%)in 72 patients with intact fibrous annulus and 32 cases(68.05%) in 47 patients with broken fibrous annulus had excellent recovery;There was significant difference in therapeutic effect existed between bulging,protrusion patients and extrusion patients. The same outcome was found between intact fibrous annulus groups and broken fibrous annulus groups. Two cases in three patients whose lumbar disc protrusion accompanied spinal stenosis had excellent recovery. No serious complication occurred. Conclusion:The therapeutic method developed by injecting O2-O3 mixture in lumbar intradiscal and paraspinal space was a safe and effective method for the treatment of the lumbar disc herniation. The therapeutic effect was fine in bulging, protrusion groups and intact fibrous annulus groups. Ozone ablation therapy might be tried in the patient with lumbar disc protrusion accompanied spinal stenosis.