Objective:To study the effect of the centrifugal force on the constructing of tissue engineering nucleus pulposus. Methods:Nucleus pulposus were isolated by trypsin and type Ⅱ collagenase from rabbit thoracic and lumbar intervertebral discs.Cells were proliferated to the third generation and seeded into PLGA scaffolds(3 × 107/ml). Experimental groups were subjected to the centrifugal force(134.16g and 301.86 g of relative-centrifugal force,twice per day,for 20 min) while the control group was cultured under normal condition,each group containing 4 specimens. The complex of nucleus pulposus and scaffolds were observed after 4 weeks. The structure of nucleus pulposus and typeⅡ collagen secretion were assessed by HE,immunohistochemistrical stain and the scanning electron microscope. Results:After 4 weeks,all groups formed the similar of complex of nucleus pulposus and scaffolds. The tissue engineered nucleus pulposus under centrifugal force were morphologically thicker than that in control,the area of immunohistochemistrical stain was(14.36 ± 2.82)% in experimental group 1,(18.71 ± 2.14) in experimental group 2,(9.13 ± 1.58)% in control group respectively. There were statistical significance between experimental group and control group(P < 0.01),also between experimental group 1 and experimental group 2(P < 0.05). Conclusion:The centrifugal force promotes nucleus pulposus cells to secrete typeⅡ collagen and the centrifugal force might benefit the construction of tissue engineered nucleus pulposus.