Objective:To investigate the relationship between activation of NF-κB,expression of livin and survivin,two members of inhibitor of apoptosis protein(IAP) family,and drug resistance in gastric carcinoma. Methods:The inhibition of proliferation and apoptosis of gastric cancer cell SGC7901and SGC7901/VCR were detected by MTT and Flow cytometry. Activation of transcription of NF-κB was detected by ELISA. Protein expression of livin and survivin were assessed by Western blot analysis. Results:The inhibition of proliferation were reduced in SGC7901/VCR cells after been exposed to VCR,and the ratio of apoptosis was also decreased. We found that NF-κB activity was enhanced,and livin,survivin were overexpressed in SGC7901/VCR cells by ELISA and Western blot assay. Conclusion:The activation of NF-κB and upregulation of livin and survivin may be involved in the process of drug resistance in SGC7901/VCR cells . Upregulation of livin and survivin might relate to activation of NF-κB.