Objective:To investigate the effects of Src signal pathway on the adhesion and migration in human breast cancer cells(MDA-MB-231) and its role in the reorganization of actin cytoskeleton. Methods:The MDA-MB-231 cells were treated with Src inhibitor PP2 in various concentrations(0.2,1.0,5.0 and 25 -滋mol/L) for 24 h. MTT assay was used to detect the adhesive ability of MDA-MB-231 cells to artificial basement membrane. Migration rate was measured by wound healing assay. To detect the reorganization of actin cytoskeleton,the contents of G-actin(in cytosolic fraction) and F-actin(in cytoskeletal fraction) in cells were evaluated by Western blot, and the distribution of F-actin stained by FITC-phalloidin in cells was also examined by fluorescence microscopy. Results:After treated with PP2 for 24 h,the ability of adhesion and migration in MDA-MB-231 cells were significantly reduced compared to the control cells in a dose-dependent manner. Consistently,the celluar F-actin was also depolymerized after PP2 treatment. Conclusion:Src inhibitor PP2 can inhibit the ability of adhesion and migration in MDA-MB-231 cells, which probably be through the reorganization of actin cytoskeleton.