Objective:To study the existence status of the integron and transposable element of multi-resistant Acinetobactor baumannii isolates in clinic in Nanjing. Methods:The samples of 20 multi-resistant acinetobacter baumannii isolates were collected from Jul, 2007 to Oct, 2007 of patients in hospitals of Nanjing. To determine the sensitivity to the 11 antibacterial a broth induction method was used, the genetic marker of integron qacE△1-sul1 and transposable element tnpU were analyzed by polymerase chain reaction(PCR). Results:In the 20 AB isolates, the rate of the positive of integron qacE△1-sull is 70%, and the rate of transposable element tnpU is 65%. Conclusion:The positive rate of the integron qacE△1-sul1 and transposable element tnpU for multi-resistant acinetobactor baumannii is high in Nanjing region. It should be reevaluated the chlorhexidine used as precaution infection drug after operation.