Objective:To investigate the protective effect of rhKD/APP on liver ischemia-reperfusion injury of rats and its mechanism. Methods:All rats were divided into 6 groups randomly, sham-operation group, model group, rhKD/APP treatment groups and Aprotinin group, 10 in each group. rhKD/APP was given with different dose in rhKD/APP treatment groups. Pathological model of liver ischemia-reperfusion injury of rats was set up. Rats were killed after 40 min ischemia and 3 h reperfusion. The levels of ALT, AST and ALP in serum were measured. Hepatic tissue was observed under light and electronic microscopy. The expression of TNF-α protein in tissue was determined by immunohistochemistry. Results:rhKD/APP can significantly decrease the activities of ALT, AST and ALP. Under light and electronic microscopy, the extent of damaged hepatic tissue were observed decreased in the groups treated with rhKD/APP. Expression of TNF-α protein in tissue was significantly reduced(P < 0.01). Conclusion:rhKD/APP has a protective effect on liver ischemia-reperfusion injury of rats. rhKD/APP can obviously inhibit the inflammatory process by inhibiting TNF-α.