Objective:To observe the effect of early nutrition in very low birth weight infants. Methods:Sixty-three preterm infants with birth weights <1 500 g received parenteral nutrition containing both 2.0 g/(kg·d) amino acids(AA) and 1.5 g/(kg·d) lipid within the first 24 hours with a increase of 1.0 g/(kg·d) in both of them(ETPN group), or solely glucose during the first day with 0.5 g/(kg·d) AA in second day and 0.5 g/(kg·d) lipid in the third day with a increase of 0.5 g/(kg·d) in both of them(ETPN group). The final dose of AA is 3.5 g/(kg·d) and lipid is 3.0 g/(kg·d). To compare nitrogen balances and caloric intake in the two groups,the plasma concentration of cholesterol,triglyceride,bilirubin,creatinine were determined to evaluate the side effect of ETPN. Results:VLBW infants supplemented with ETPN had no major adverse side effects. They got a positive nitrogen balance at the first day and more calories intake. Conclusion:Early total parenteral nutrition administration to VLBW infants results in a positive nitrogen balance and more calories intake.