Objective:To investigate the effect of resveratrol on the density of L-type calcium current(ICa-L) in cultured guinea pig ventricular myocytes. Methods:Single cultured ventricular cells were obtained from healthy adult male guinea pig heart using enzymatic dissociation methods. The whole-cell patch clamp was used to record the ICa-L after administration of resveratrol at 24 h and 72 h with different final concentration of 0,10,30,100 μmol/L. Results:①30 μmol/L and 100 μmol/L RES decreased the density of ICa-L by 13% and 27%,respectively,at 72 h. There is a significant difference between control group and RES 100 μmol/L group(P < 0.05);②RES 10 μmol/L,30 μmol/L and 100 μmol/L have a tendency of reducing the density of ICa-L in a time-dependent manner. 100 μmol/L RES reduced ICa-L more significantly at 72 h compared with that did at 24 h(P < 0.05). Conclusion:Resveratrol have a tendency of inhibiting the density of L-type calcium current in cultured guinea pig ventricular myocytes at a concentration-and time-dependent manner.