Objective:To investigate the expression levels of MRP1,LRP and BCRPmRNA in patients with non-small cell lung cancer(NSCLC) as well as their clinical prognostic significance. Methods:RT-PCR method were used to detect the expression levels of MRP1,LRP and BCRPmRNA in tissues samples and corresponding adjacent normal lung tissues of 60 patients with NSCLC. T-Test,One-Way ANOVA,rank correlation,Kaplan-Meier survival curve,and COX multivariate regression analysis were used for statistical analysis. Results:The expression levels of MRP1and LRPmRNA were higher in NSCLC tissues than that in normal lung tissues(P = 0.02 and P = 0.000). There were significant correlations between intratumoral expressions of MRP1mRNA and LRPmRNA(rs = 0.530,P = 0.000). The expression level of BCRPmRNA was lower in NSCLC tissues than that in normal lung tissues(P = 0.042).LRPmRNA expression level was higher in lung adenocarcinomas than in squamous carcinoma(P = 0.02). None of them were related to clinical TNM stage. The patients with low expression of MRP1mRNA had significantly longer survival time than those with high expression of MRP1mRNA(P = 0.023). COX multivariate regression analysis showed that expression of MRP1mRNA was an independent prognostic factor for NSCLC. Conclusion:MRP1,LRP and BCRPmRNA were expressed in various degrees in NSCLC tissues.The expression level of MRP1mRNA was related to overall survival time of NSCLC. MRP1mRNA expressions can be used as a prognostic predictor for NSCLC.