Objective:To investigate the mental health status,cigarette smoking,alcohol drinking and their relationship among medical staff. Methods:The 1 418 medical staff in 7 comprehensive hospitals of Jiangsu province were investigated using symptom check list 90(SCL-90) and a self-designed questionnaire. Data were input to create a database with EpiData 3.02 and analyzed with SPSS 13.0 software package. Results:Total 1 447 questionnaires were sent out and 1 418 qualified ones were collected, and the effective response rate was 98.0%. There were 30.5% male and 69.5% female,with the mean age 35.6 ± 11.1 years. This survey showed that the total smoking rate was 10.0%,with 31.2% in male and 0.8% in female. The standardized total smoking rate was 11.7% according to the national population proportion by age in 2000,with 26.6% in male and 1.3% in female. Smoking rate in male was significant higher than in female,especially in the medical technician(25.8%). The total drinking rate was 14.0%,with 40.4% in male and 2.6% in female. The standardized total drinking rate was 16.3%,with 37.0% in male and 1.4% in female. Drinking rate in male is more significant than that in female,especially in the medical technician(28.9%). Comparing the smokers and the non-smokers,there were 3 factors(hostility,paranoid ideation and psychoticism) without significance,while others(somatization,obsession-compulsion,interpersonal sensitivity,depression,anxiety,phobic anxiety) of the smokers were all significantly higher than the non-smokers. According to whether they drank or not,the medical staff were divided into two groups. The result revealed that there were significant differences in the mean scores of somatization and phobic anxiety between the two groups,while other 7 factors(obsession-compulsion,interpersonal sensitivity,depression,anxiety,hostility,paranoid ideation and psychoticism) had no significance between the two groups. Conclusion:Drinking and smoking rate of the medical staff in comprehensive hospitals were lower than that of general population,while the smokers of medical staff had lower metal health level.