Objective:To study the growth inhibition effect and its molecular mechanism of SGC-7901 cells by the tumor suppressor gene hING4 in vitro. Methods:The hING4 gene was transfected into human gastric carcinoma cell line SGC-7901 with a replication-incompetent adenovirus vector. The mRNA transcriptions of hING4 in SGC-7901 cells was confirmed using RT-PCR. The anti-tumor efficacy of hING4 on SGC-7901 cells was evaluated by MTT,and cell apoptosis were detected by Hoechest 33258 staining and laser scanning confocal microscopy(LSCM). The cell cycle and distribution and apoptotic peak was evaluated by flow cytometry. Bcl-2 and Bax were explored by means of immunocytochemistry. Results:hING4 was proved successful transcription in SGC-7901 cells.hING4 can obviously inhibit proliferation and induce S phase reduction,G2/M phase arrest and cell apoptosis of SGC-7901. hING-4 can also down-regulate the expression of bcl-2 and up-regulate the expression of bax. Conclusion:hING4 can obviously inhibit proliferation and induces cell apoptosis of SGC-7901 cells. Down-regulating the expression of bcl-2 and up-regulating the expression of bax may be one of the molecular mechanisms involved in the anti-tumor effect.