Objective:To prepare clobetasol propionate liposome, investigate its stability and study its pharmacodynamics on guinea pig eczema model. Methods:Clobetasol propionate liposome was prepared with revolution thin film dispersion. The stability of indices,such as the content of the principal agent,grain size,encapsulation efficiency,was investigated. The pharmacodynamics of different preparation on guinea pig eczema model were studied by evaluating cellular infiltrates in dermis. Results:The content of the principal agent,grain size and encapsulation efficiency of liposome were(100.84 ± 2.0)%,(140.5 ± 56.8)nm,(90.1 ± 2.3 )%,respectively. No obvious changes were found in all the indices in the stability of liposome under three different storage condition.There was significant difference in cellular infiltration in dermis of CP liposome group and commercial CP cream group. Conclution:The preparation was proved to be of high encapsulation efficiency,good stability and better curative effect than cream.