Objective:To obtain recombinant HPV16 E6 specific RNA interference expressi-on vectors and investigate the inhibitory effects of the vectors on the expression of human papil-omavirus E6 gene. Methods:According to the computer aided design,56nt oligonucleotide fragments containing different HPV16 E6 specific sequences were synthsized and cloned into the expression vectors,The recombinants were transfected into cervical cancer cell line,Caski,with liposomes. Expression of E6 was detected by FQ-PCR(fluorescence quantitative PCR) and flow cytometry. Results:HPV16 E6 specific siRNA expression vectors were confirmed by PCR analysis and DNA sequencing. Three kinds of expression vectors could reduce the expressions of E6 mRNA and protein all in Caski-B cell. The inhibition ratio of E6 mRNA reduced to 89.5%, and protein inhibition ratio reached 98.1%. Conclusion:The RNAi expression vectors can effectively inhibit the expression of HPVE6 Gene.