Objective:To modify the manipulation of CD11c cells magnetic beads sorting,and explore the effect of CD11c cells on inducing the proliferation of autogenic CD4+T and CD8+T cells. Methods:Collagenase,DNaseⅠand EDTA were used for digesting the spleen tissue,and murine serum and CD16/32 antibody were applied for blocking the nonspecific connecting of CD11c beads and the spleen cells. After modified beads sorting,the purity of CD11c cells was checked by flow cytometry. The proliferation of autogenic CD4+T and CD8+T cells and the concentration of cytokines in the supernatant of the cocultue cells were detected by mixed lymphocyte culture and ELISA,respectively. Results:Average(4.52 ± 0.05)×106 CD11c cells were collected in each murine spleen by the modified beads sorting,and the purity of CD11c was 98%. The proliferation of CD4+T and CD8+T cells and the secretion of IL-2 and IFN-γ of these cells were significantly induced by CD11c cells of recombinant E.coli LLO/OVA vaccinated mice. Conclusion:More quantity and higher purity of CD11c cells were collected by modified magnetic beads sorting,and the actived CD11c cells play a role on inducd autogenic T cells proliferation and cytokines secretion.