Construction of human spasmolytic polypeptide expression vector and inductively expression in E.coli
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    Abstract:

    Objective:To construct E.coli expression vector of human spasmolytic polypeptide(hSP),express recombinant hSP and underlie the base of function analyses. Methods:The hSP gene encoding mature peptide was amplified by reverse transcriptase polymerase chain reaction,and then inserted into the expression vector pET32a. Recombinant plasmid pET32a-hSP was transformed into the E.coli Origami B(DE3). Recombinant proteins were assayed with SDS-PAGE and Western blot. Results:It was proved that the fragment amplified was inserted into the expression vector pET32a correctly by PCR and gene sequencing. Tricine SDS-PAGE analyse proved that the molecular weight of hITF was about 32 kD and Western blot demonstrated that the expression proteins have much good antigenicity and specificity. Conclusion:hSP E.coli expression vector was successfully constructed and recombinant hSP was expressed. This research lay foundations for the further function studying of hSP.

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孙勇,孙曙光,吴炜,张勇,吕尚军,王林,彭曦,汪仕良.人解痉多肽原核表达载体的构建及诱导表达[J].南京医科大学学报(自然科学版英文版),2008,28(12):1537-1540.

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  • Received:June 20,2008
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