Objective:To study the character of supratrochlear artery(STA) with ultrasonography and anatomy and its application in designing forehead flap. Methods:A total of 30 STAs of 15 normal adults were detected by ultrasound and 12 STAs of 7 adult cadavers were dissected to study their calibers,courses and the relationship to the surrounding soft tissue. Results:Thirty STAs of normal adults and 12 STAs of cadaver controls were displayed well. The STA where it started in normal adults was(1.20 ± 0.17)mm in outer diameter and(1.40 ± 0.16)cm away from the midline of the face,and in adult cadaver controls was(1.28 ± 0.22)mm and(1.33 ± 0.14)cm. Twenty-six STAs in normal adults and 10 STAs in cadavers ascended inward. There was no statistial difference between the two groups(P > 0.05). Conclusion:The position of STA was permanent but some variation,which can be clearly displayed by ultrasonography. Ultrasonography is a good method to help design the forehead flap.