Objective:To establish a HPLC method for the determination of ginsenoside Rg1, Re and Rb1 in Yihechun Oral Solution. Methods:The separation was performed by gradient elution on a Shim-pack VP-ODS(150 × 4.6 mm) with the mobile phase A:acetonitrile-methyl alcohol-0.3% Phosphoric Acid solution(10 ∶ 5 ∶ 85) and the mobile phase B:acetonitrile-methyl alcohol-0.3% Phosphoric Acid solution(50 ∶ 5 ∶ 45). The monitoring wave length was set at 203 nm. Results:Ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were in good linearity between 0.04 and 0.4 mg/ml. The results showed that average recovery rate were 96.0 ± 3.2%, 94.1 ± 1.6%, 96.2 ± 3.3% and stability test RSD were 1.19%-2.85%-3.17% respectively,the RSD of the replicate test were 1.56%-1.97%-3.29%, respectively. Conclusion:The HPLC method is suitable for the quality control of Yihechun Oral Solution.