Objective:To observe the long-term effect of mitomycin C combined with Ahmed glaucoma valve(AGV) implantation on the refractory glaucoma. Methods:After A GV implantation, a retrospective study of clinical effect and complications were conducted on 35 eyes from 32 patients with refractory glaucoma with one-year follow-up. Results:The long-term successful rate was 80.0%. The average IOP decreased from the preoperative(47.28 ± 13.14)mmHg to the postoperative(17.62 ± 6.42)mmHg. The complications caused by the unsuccessful operation included bleb encapsulation in 2 eyes(5.7%), flat anterior chamber in 2 eyes(5.7%), tube blockage in 2 eyes(5.7%), and exudativech roidal detachment in 1 eye(2.8%). Conclusion:Mitomycin C combined with the AGV implantation surgery may be effective and safe for treating refractory glaucoma.