Objective:To study the expression of VDR protein in the femurs of offsprings whose mothers were deficient of vitamin D. Methods:Thirty-six adult female SD rats were chosen and divided randomly into the control group(18 rats) and model group(18 rats).The control group was received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks. Then the female rats in two groups copulated with the male normal rats. The left femurs were collected from the 20-day embryos and the 1-day and 7-day newborns. VDR protein expression was measured by Western blotting. Results:The VDR protein expression of the model group was lower than the control group(P < 0.05). Conclusion:The change of expression of VDR protein was due to vitamin D deficiency during the pregnancy, which may be the reason of offsprings’ skeletal deformity.