Objective:To evaluate the property of IgG autoantibody assay protein chip in detecting anti-ENA autoantibody profiles. Methods:The sera of 249 patients were collected. The samples were detected with IgG autoantibody assay protein chip in comparison with immuloblotting test. Results:IgG autoantibody assay protein chip can detect anti-ENA autoantibody profiles in serum of patients. In comparison with immunoblotting assay, the coincidence rate was 82.2% for anti-SS-A/Ro, 88.2% for anti-SS-B/La, 94.7% for anti-Scl-70, 95.9% for anti-Jo-1, 92.5% for anti-Sm, 88.5% for anti-ulRNP, 82.2% for anti-Rib-P, respectively. Conclusion:IgG autoantibody assay protein chip can be used to detect anti-ENA autoantibody profiles from patients serum. There is no significant difference between detection results from IgG autoantibody assay protein chip and that from immunblotting test.