Objective:To investigate the effect of movement training on the motor function repair of rats with a spinal cord contusion, and to simultaneously observe BDNF, Slit2 and GFAP expression of rats. Methods:Seventy male adult Sprague Dawley rats were randomly divided into 10 training groups and 4 control groups,each group with 5 rats. Moderate spinal cord contusive injuries were produced by using modified Allen’s impactor method to all experimental rats. After injury,rats in training groups were exposed to the treadmill locomotion test for 1 to 4 weeks. The behavioral scores were recorded and the expression of BDNF, Slit2 and GFAP in spinal cord were detected immunohistochemistry at different time point(14, 21, 28, 35 days after concusion). Results:Compared with the control groups, the behavioral scores improved significantly after 1 to 4 weeks exercise(P < 0.05),and a significant increase of BDNF,Slit2 in the spinal also were observed in exercise training groups(P < 0.05). The expression of GFAP delayed reached the peak level. Conclusion:The results indicate that rehabilitation training may play an important role in nerve functional recovery and the mechanism maybe promote the expression of BDNF and Slit2, delay hyperplasia of glial cell and weaken the inhibitory factor of neuron recovery.