Objective:To establish modles of rat ischemia preconditioning(I/P) and ischemia reperfusion(I/R) and to study the changes of I kappB kinaseβ(IKKβ) and Neutrophil Gelatinase-Associated Lipocalin(NGAL). Method:Thirty male Sprague-Dawley rats were divided into 3 groups randomly after right kidney nephrectomy. Group A was sham-operated control. Group B rats were 45-minutes ischemic in the left renal artery while Group C rats were pretreatmented with 3 cycles of 2-minutes ischemia and 5-minutes reperfusion. All the rats were sacrificed at 24 h after reperfusion. We harvested kidneys and serum to do further analysis, including histological and functional parameters,expressions of NGAL and IKKβ in renal tissues. Results:Compared with rats suffered from ischemia-reperfusion injury, pretreatmented rats had a significant decrease in serum creatinine level and tubulointerstitial injury scores. In addition, expressions of IKKβ and NGAL at renal tubule were attenuated significantly. Moreover, our study showed IKKβ and NGAL were in positive correlation. Conclusion:IKKβ plays roles in renal ischemia-reperfusion injury ,give rise to the generation of NGAL. IKKβ inhibition in ischemia preconditioning attenuates renal injury and the espression of NGAL following acute ischemia-reperfusion injury