Objective:To estimate whether PAPP-A(Pregnancy-associated plasma protein-A) polymorphism is associated with pre-eclampsia. Methods:By means of polymerase Chain Reaction and Single-Strand Conformation Polymorphism,S1224Y(A/C)(dbSNP: rs7020782) polymorphism in the PAPP-A gene was detected in 129 women with pre-eclampsia(64 with mild pre-eclampsia and 65 with severe pre-eclampsia) and 137 normal control women with uncomplicated pregnancy. Investigators were blinded to clinical outcomes. Results:AC genotype frequency in pre-eclampsia group were higher than those in control group(44.2% VS 31.4%)(P﹤0.05); AC genotype frequency in severe pre-eclampsia group were significantly higher than those in mild pre-eclampsia group (75.4% VS 12.5%)(P﹤0.001). C allele frequency in severe pre-eclampsia group were significantly higher than those in mild pre-eclampsia group(40.8% VS 18.7%)(P﹤0.001). Conclusion:The AC genotype and C allele of S1224Y(A/C) polymorphism in the PAPP-A gene might be a risk genetic marker of pre-eclampsia. In patients with pre-eclampsia,there is the association between AC genotype and C allele of PAPP-A gene and severity of pre-eclampsia.