Objective:To investigate the effect of PGE2 on the expression of Survivin in HuH7 cell line and its correlation with PI3K/AKT signal conduction pathway. Methods:HuH7 cells were treated with PGE2,Celecoxib(EP receptor agonist),LY294002(EP receptor inhibitor). Western blotting was employed to detect the expression of Survivin in HuH7 cells. Results:The expression of Survivin in HuH7 cells were increased by 63.59%,114.76%,76.68% and 70.01%(P < 0.01)after treatment of PGE2(5 μmol/L) and EP1,EP3,EP4 receptor agonist(5 μmol/L)for 8 h,and decreased by 32.95%(P < 0.01)and 28.36%(P < 0.05) after treatment of EP1 and EP4 receptor inhibitor(10 μmol/L)for 8 h respectively,but have no change after treatment of EP2 receptor agonist(5 μmol/L) and EP2 receptor inhibitor(5 μmol/L)(P > 0.05). After the treatment of LY294002(10 μmol/L) for 8 h,the expression of Survivin in HuH7 cells were decreased by 28.05%(P < 0.01) vs that treated with PGE2 . Conclusion:Our findings suggested that PGE2 might up-regulate the expression of Survivin through EP1,EP3 and EP4 receptors,which could be partly related to the PI3K/AKT signal conduction pathway.