Objective:To determine whether bone marrow mesenchymal stem cells(BM-MSCs) can migrate subcutaneously into different organs. Methods:The bone marrow cells isolated from β-galactosidase transgenic mice were cultured. BM-MSCs were passaged until the 3rd generation. The 3rd passaged BM-MSCs derived from β-galactosidase transgenic mice were transplanted into 6-week-old C57BL/6J wild type mice subcutaneously. 8 weeks later,β-gal+ MSCs were shown the distribution in various organs from recipient mice through LacZ staining. The expression of matrix metalloproteinase(MMPs) genes in the 3rd passaged β-gal+ BM-MSCs were examined by real-time RT-PCR. Results:The 3rd passaged BM-MSCs derived from β-galactosidase transgenic mice were positive for LacZ staining. Followed subcutaneous injection of the 3rd passaged β-gal+ BM-MSCs for 8 weeks,β-gal positive organ specific cells were detected in lung,brain,heart,liver and kidney from recipient mice. MMPs genes were expressed in the 3rd passaged β-gal+ BM-MSCs. Conclusion:BM-MSCs transplanted through subcutaneously can migrate into various organs through blood circulation and may differentiate into various organ specific cells to participate in self-renewal of normal tissues.