Objective:To obtain the PANC1 pancreatic cell line which was stably transfected with HCCR siRNA plasmid and investigate the effect of proliferation and invasion on PANC1 cell by knockdown of HCCR expression through RNA interference. Methods:The siRNA targeting HCCR was designed and the plasmid of pGCsi-HCCR was synthesized. Plasmid of pGCsi-HCCR was transfected into PANC1 cells. The vector pGCsi was used as the negtive control. G418-resistant clone was selected and obtained. Proteins of the stable-transfected cell lines was detected by western blot using HCCR antibody. p53 protein in the stably transfected cell lines was also detected. Flow cytometry(FCM), MTT and Transwell assay were examined to observe the apoptotic rate, proliferation and invasion of HCCR siRNA transfectant. Results:Plasmid of pGCsi-HCCR can inhibit the expression of HCCR. The protein level of P53 was decreased in HCCR siRNA cells compared with the vector cells. Plasmid of pGCsi-HCCR can induce a more significant G0/G1 stage arrest and higher apoptotic rate compared with control cells. The proliferation of HCCRsiRNA cells were decreased by 0.65 times and 0.68 times in HCCRsiRNA cells compared with control cells. The number of invasion cells were 24.4 ± 9.9 and 49.1 ± 15.4 respectively, which were significant difference. Conclusion:Down-regulating the expression of HCCR gene by siRNA can inhibit proliferation and invasion and induce apoptosis in PANC1 cells.