Objective:To investigate the protective effect of PEG1 and adenosine perfusion on the isolated lung of rabbit. Methods:Eighteen rabbits were randomly divided into 3 groups which contain control group(A),UW fluid with PGE1 group(B),UW fluid with adenosine group(C). The lungs and hearts of rabbits were dissociated as the isolated lung and heart model. The model was perfused with low temperature perfusion fluid and conserved. The lung specimens were obtained in order to analyze the changes in morphology by using electron microscope at 2 and 4 h after perfusion. The ratio of wet/dry weight(W/D),airway pressure(Paw)of lung model,gas analysis of the blood which was pumped into the lung model through main pulmonary artery were detected,and then the blood which was elicited from left atrium pulmonale by using a vacuum aspiration tube after 4 hours of perfusion was collected. Results:The protective effects on the gas exchange function of the lung and the moisture capacity in group B were better than those in group A and C significantly. The protective effect on Paw,moisture capacity and the gas exchangs function of lung of group C was better than those of group A. Conclusion:Perfusion of the isolated lung with PGE1 can get longer time of preservation and better function of lung tissue gas exchange than pertusion with UW solution.