Objective:To explore the role of mineralocorticoid receptor(MR) in peroxisome proliferator-activated receptor γ(PPARγ)promoting white adipose cell differentiation. Methods:With the process of adipose differentiation,3T3-L1 fibroblasts were long-term exposure to pioglitazone,an agonist of PPARγ. The mRNA levels of MR and relative genes were detected by real-time quantitative PCR. Results:Accompanied with pioglitazone induced adipogenesis,mRNA levels of MR and 11β-hydroxysteroid dehydrogenase type 1 were increased. Conclusion:PPARγ agonist promoted the gene expression of mineralocorticoid receptor in adipocyte differentitation.