Objective:To evaluate the effects of Formoterol(β2-adrenergic receptor-specific agonist) and ICI118551(β2-adrenergic receptor-specific antagonist) on rat osteoblast-like cells induced from marrow-derived mesenchymal stem cells(MMSC). Methods:MMSCs by whole bone marrow adherent culture derived from a three-week-old female SD rat are induced to osteoblast-like cells. After different concentrations of Formoterol and ICI118551 are added into the culture medium,thealkali phosphatase and osteocalcin is detected. Results:The alkali phosphatase,osteocalcin and proliferation of cells cultured with all different concentrations(10-5-10-9 mmol/L) of Formoterol are obviously low. As to ICI118551,high concentrations(10-5 and 10-6 mol/L) can inhibit the expression of the alkali phosphatase,osteocalcin and proliferation of cells while low concentrations(10-8 and 10-9 mol/L) can enhance the expression of the alkali phosphatase,osteocalcin and proliferation. Conclusion:The β2-adrenergic receptor-specific agonist can inhibit the activity of rat osteoblast-like cells in vitro while the effects of the β2-adrenergic receptor-specific antagonist are not single,depending on its concentration.