Objective:To explore the antitumor effect of resveratrol against malignant melanoma in vitro and in vivo. Methods:Murine B16 and human A375 cells were cultured in vitro and the growth inhibition effects of resveratrol were detected by MTT method. After the pretreatment with different doses of resveratrol,cell protein was extracted and the expression levels of protein p-Akt were detected by Western blot. B16 cells transplant model was established in male ICR mice and the tumor growth inhibition effects induced by resveratrol were detected by measuring tumor volume every two days. Results:Cytotoxicity test showed that cell growth was significantly inhibited by resveratrol. Moreover,resveratrol effectively downregulated the expression of protein p-Akt. Animal experiments also demonstrated that resveratrol efficiently inhibited tumor growth without obvious bodyweight change. Conclusion:resveratrol was demonstrated as a potential anti-melanoma agent both in vitro and in vivo with the possible mechanism of downregulation of p-Akt expression. The current study provided the experimental basis for the clinical application of resveratrol against malignant melanoma and also offered a detailed strategy for developing the antitumon effect of Chinese herbal medicines.