Objective:To investigate the effect of insulin-like-growth factor-1(IGF-1) on expression of pituitary tumor transforming gene(PTTG)in C6 cells of glioma. Methods:Glioma C6 cells were divided into four groups and treated with different concentration of IGF-1 for 24 h respectively(A:0 ng/ml;B:0.1 ng/ml;C:1.0 ng/ml;D:10.0 ng/ml). PTTG mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR),Western blot was used to detecte the expression of PTTG protein. Results:The expressions of PTTG mRNA and protein increased dramaticly in the groups treated with IGF-1,and there was a significantly difference between each each groups(P < 0.01). Conclusion:IGF-1 can upregulate the expression of PTTG significantly in dosage-dependent manner.