Objective:To investigate the effect of basic fibroblast growth factor(FGF-2) on pim-3 in humanl lung cancer cell A459. Methods:Lung cancer cell A459 was treated by different concentrations(0-25-50-100 ng/mL) of FGF-2,pim-3 mRNA was measured by reverse transcription polymerase chain reaction(RT-PCR),and western blotting was used to detect the expression of PIM-3 protein. Results:The expressions of pim-3 mRNA and protein increased dramaticly in the groups treated with FGF-2 compared with conorol,and there was a significantly difference between each two experiment groups(P < 0.01). Conclusion:FGF-2 can significantly upregulate the expression of pim-3 in dosage-dependent manner.