Objective:To investigate the effects of lipoxin A4(LXA4)on epithelial- myofibroblast transdifferentiation(EMT)induced by recombinant human connective tissue growth factor(CTGF)in human renal tubular epithelial cell line(HK2)in vitro. Methods:The cultured HK2 cells were stimulated with CTGF for 48 hours with or without pretreatment of LXA4. The morphological changes were observed under an inverted microscope. The expressions of E-cadherin,α-smooth muscle actin(α-SMA)were detected by RT-PCR and Western blot. Results:CTGF induced the morphological changes of HK2 cells from oval to fusiform and stellate appearance,down-regulated the E-cadherin expression and up-regulated α-SMA expression both on mRNA and protein level. The expressions of mRNA and protein of E-cadherin reduced by CTGF(0.067 ± 0.015 and 0.257 ± 0.221,respectively)were increased by LXA4(0.270 ± 0.082 and 0.827 ± 0.177 respectively,P < 0.05). The expressions of mRNA and protein of α-SMA stimulated by CTGF(0.653 ± 0.256 and 0.167 ± 0.050,respectively)were inhibited by LXA4(0.070 ± 0.046 and 0.023 ± 0.023,respectively,P < 0.05). Conclusion:LXA4 inhibited the EMT of HK2 cells induced by CTGF. It suggests that the inhibition of LXA4 on CTGF-induced EMT could be a therapeutic strategy against tubulointerstitial fibrosis.