Objective:To evaluate expression of Fgfs in hypopspadiac male rats induced by maternal exposure to DBP. Methods:Twenty pregnant rats were randomly divided into 2 groups and given DBP by gastric intubation at a dose of 0,750 mg/kg from gestation day(GD)14 to GD18. On postnatal day(PND)1,live pups were counted and hypospadias of male pubs were examined. The anogenital distance(AGD)and body weight of male pubs were measured. On PND7,real-time quantitative RT-PCR was used to quantify mRNA expression of Fgf8,Fgf10 and Fgfr2 in GT of hypospadiac male rats. Results:The number of live pubs in the DBP treated group significantly decreased. The body weight and the AGD of hypospadiac male rats significantly decreased. Moreover,significantly decreased expression of Fgf8,Fgf10 and Fgfr2 were observed. Conclusion:The down-regulation expression of Fgfs signaling molecules might lead to the occurrence of hypospadias induced by maternal exposure to DBP.