Objective:To analyze the subtypes distribution of Human Immunodeficiency Virus-1(HIV-1)in Jiangsu province,and the relationship between subtypes and transmission route. Methods:83 blood samples with EDTA-K3 were collected from population with HIV-1 confirmed lately using western blot test. Viral RNA was extracted from plasma and used by a RT-nest PCR to amplify the env and gag gene of HIV-1. The PCR amplimers were partially sequenced on ABI 3730. After sequences alignmented by ClustalX,phylogenetic analysis was performed using MEGA 3.1 software. Results:7 subtypes including CRF01_AE,CRF07_BC,CRF08_BC,B,C,CRF02_AG,A1 were found circulating in Jiangsu. 3 predominated clades were CRF 01_AE,CRF07_BC,and B,which accounted for 31.6%,25%,and 19.7%,respectively. 5 subtypes consisting of CRF07_BC,B,CRF01_AE,C,CRF08_BC,circulated in population via heterosexual contact,and CRF 07_BC accounted for 32.6%,B was 23.3%,and CRF 01_AE was 20.9%. CRF 01_AE,CRF 07_BC,B,CRF 08_BC circulated among men who sex with men having HIV-1,and CRF 01_AE was dominative. The circulated HIV-1 subtypes among IDUs included CRF 07_BC-B-CRF 01_AE-A1-CRF 02_AG,CRF 01_AE was the dominated,which accounted for 58.4%. Conclusion:There were more kinds of subtypes circulating in Jiangsu than other province. Of 7 subtypes,5 except CRF02_AG and A1 were found in population with HIV-1 via heterosexual contact. The increasing complex characteristic of HIV-1 sequences may be associated with acquiring HIV via sexual intercourse.