Objective:To evaluate the effects of implementation of 3-years’s HIV/AIDS behavior intervention program, and provide evidence for developing intervention strategies. Methods:Two counties in every south, middle and north part of Jiangsu province were selected as representative, used same approach of two-stage cluster sampling to recruit entertainment based female sex workers (FSWs). Questionnaires survey,blood and cervical secretion collection were conducted in 2006 and 2008 respectively. Results:FSWs selected came from similar group during the two cross investigation. After intervention, the awareness rate of AIDS related knowledge increased from 69.2% to 89.4%, and the condom use rate in the previous sexual intercourse increased from 72.5% to 90.2%. The self-protection consciousness of FSWs got to be reinforced, and stigma and discrimination behaviors towards AIDS reduced. The prevalence rate of Syphilis was 4.4% in 2008, a little bit lower than in 2006. While the prevalence rate of Chlamydia trachomatis declined dramatically from 23.1% in 2006 to 10.7% in 2008. The occurrence of incorrect behaviors, for example, immediately vaginal douching after sexual intercourse or took antibiotics to prevent AIDS/STD reduced continuously. In multivariate Logistic analysis, the factors associated with condom use were: registration in Jiangsu province, high number of clients, and good skills at refusal and negotiation to clients. AIDS knowledge looked no statistical association with the condom use. Conclusion:The implementation of scaling up HIV/AIDS behavior intervention program was proved to be positively effective in Jiangsu.