Effect of JAK/STAT signaling pathway on extracellular release of HMGB1 in lipopolysaccharide-induced hepatocytes
DOI:
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective:To study the effect of JAK/STAT signaling pathway on extracellular release of high mobility group box 1 (HMGB1) in endotoxin-induced hepatocytes. Methods:HMGB1 concentration was determined in the culture medium of 100 μg/L lipopolysaccharide-induced BRL-3A hepatocytes by enzyme-linked immunosorbent assay (ELISA),and the change of HMGB1 mRNA expression was detected by RT-PCR method. The effects of JAK/STAT pathway inhibitors (tyrphostin AG 490 and fludarabine) with various concentrations on HMGB1 mRNA expression and extracellular release were investigated. Results:The level of HMGB1 mRNA expression and HMGB1 concentration in the culture medium significantly increased after BRL-3A hepatocytes induced by lipopolysaccharide for 24 hours(P < 0.01). 25 μmol/L tyrphostin AG 490 and 50 μmol/L fludarabine partly inhibited HMGB1 mRNA expression and extracellular release in lipopolysaccharide-induced BRL-3A hepatocytes(P < 0.05). Conclusion:Lipopolysaccharide induces hepatocytes to release HMGB1,which is related to intracellular JAK/STAT signaling pathway.

    Reference
    Related
    Cited by
Get Citation

张〓健,帅朝霞,胡志刚,王春新,袁劲松,陈国千. JAK/STAT通路在脂多糖诱导肝细胞HMGB1释放中的作用[J].南京医科大学学报(自然科学版英文版),2009,29(11):1514-.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:June 01,2009
  • Revised:
  • Adopted:
  • Online:
  • Published:
Article QR Code