Objective:To select epitopes of human inhibin subunits for fusion expression and antibody preparation according to the features of α,βA and βB. Methods:Based on amino acids sequences of inhibin subunits α(1-32),βA(82-114) and βB(101-134),the genes fragments were synthesized using preferred codons,which was inserted into pGEX-4T-2,and expressed in E. coli. The protein was purified for immunization of sheep antibody preparation. The titer of antibodies were determined by ELISA. Results:The positive clones named pGEX-INHA,pGEX-INHBA,pGEX-INHBB were choiced using enzyme digestion. The products of dones:GST-INHA,GST-INHBA,GST-INHBB,and the purified protein were identified with Commassie Blue Staining,then used to sheep immunization. The results showed that the titer of antibodies were 1∶64,1∶128 and 1∶32 using GST-INHA,GST-INHBA,and GST-INHBB,respectively,and 1∶32,1∶32 and 1∶64 using purified GST-INHA,GST-INHBA,and GST-INHBB,respectively. Conclusion:These antibodies can be used as diagnostic reagent of enzyme immunoassay detecting serum inhibin level.