Objective:To study the expression of BMP-2,Cbfa1 and Dmp1 on the femurs of offspring,whose mother is deficient of vitamin D during pregwanoy. Methods:36 adult female SD rats were chosen and allocated randomly into control group(18 rats) and model group (18 rats). The control group received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks. Then the female rats in two groups copulated with normal male rats. The left femurs were collected from the 20-day embryos(E20 d),the 1-day(B1 d) and 7-day(B7 d) newborns respectively. The expressions of bone development-related genes BMP-2,Cbfa1 and Dmp1 were measured by Real-Time PCR. Results:The expressions of BMP-2,Cbfa1 and Dmp1 mRNA in the model group were lower than those of the control group on E20d,B1d and B7d,respective (P < 0.05). Conclusion:The changes of expressions of bone development-related genes BMP-2,Cbfa1 and Dmp1 on the femurs of offspring are due to vitamin D deficiency of mother during the pregnancy,which would affect the offspring’s bone development.